Centrifuge Rpm And Rcf Calculator

Centrifuge Equations:

\[ RCF = 1.118 \times 10^{-5} \times r \times (RPM)^2 \] \[ RPM = \sqrt{\frac{RCF}{1.118 \times 10^{-5} \times r}} \]

Calculated RPM:

Calculated RCF:

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1. What is Centrifuge RPM and RCF?

RPM (Revolutions Per Minute) and RCF (Relative Centrifugal Force) are two key parameters in centrifugation. RPM refers to the rotational speed of the centrifuge rotor, while RCF represents the actual gravitational force applied to the sample, measured in g-force.

2. How Does the Calculator Work?

The calculator uses the following equations:

\[ RCF = 1.118 \times 10^{-5} \times r \times (RPM)^2 \] \[ RPM = \sqrt{\frac{RCF}{1.118 \times 10^{-5} \times r}} \]

Where:

\( RCF \) — Relative Centrifugal Force (g)
\( RPM \) — Revolutions Per Minute
\( r \) — Rotor radius (meters)
\( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: These equations allow conversion between RPM and RCF based on the rotor radius. RCF provides a more accurate measure of the actual force applied to samples.

3. Importance of RPM and RCF Calculation

Details: Accurate calculation of RCF is essential for reproducible centrifugation protocols. Different applications require specific g-forces for optimal separation of particles based on size, density, and shape.

4. Using the Calculator

Tips: Enter the rotor radius in meters, then provide either RPM or RCF value. The calculator will compute the missing parameter. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why is RCF more important than RPM?
A: RCF represents the actual gravitational force applied to samples, which is what affects separation. Different centrifuges with the same RPM but different rotor radii will produce different RCF values.

Q2: How do I measure rotor radius?
A: Rotor radius is typically measured from the center of the rotor to the bottom of the sample holder when it's in the horizontal position.

Q3: Can I use this calculator for any centrifuge?
A: Yes, as long as you know the rotor radius. The equations are universal for all centrifuges.

Q4: Why do protocols specify RCF instead of RPM?
A: Protocols specify RCF to ensure consistent results across different centrifuges and rotors, as the same RCF will produce equivalent separation regardless of equipment.

Q5: What's the typical range for centrifuge speeds?
A: Centrifuge speeds range from a few hundred RPM for low-speed applications to over 100,000 RPM for ultracentrifuges, producing RCF values from hundreds to over 1,000,000 g.