RCF Formula:
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The RCF (Relative Centrifugal Force) calculation converts RPM (revolutions per minute) to g-force, providing a standardized measure of centrifugal force independent of rotor size. This is essential for reproducible centrifugation protocols in laboratories.
The calculator uses the RCF formula:
Where:
Explanation: The formula squares the RPM, multiplies by the radius, and divides by the conversion factor to calculate g-force.
Details: Accurate RCF calculation ensures consistent centrifugation conditions across different centrifuges and rotors, which is critical for reproducible experimental results in biochemistry, molecular biology, and clinical laboratories.
Tips: Enter RPM in revolutions per minute and radius in centimeters. Both values must be positive numbers. The calculator will compute the corresponding RCF value in g-force units.
Q1: Why convert RPM to RCF?
A: RCF provides a standardized measure of centrifugal force that accounts for both rotational speed and rotor radius, allowing for consistent protocols across different equipment.
Q2: What is the significance of the 111800 constant?
A: This constant incorporates gravitational acceleration and unit conversion factors to transform RPM and radius measurements into g-force units.
Q3: How do I measure the radius accurately?
A: Measure from the center of the centrifuge rotor to the bottom of the sample tube when it's in the horizontal position during rotation.
Q4: Are there different formulas for different units?
A: Yes, the formula may vary slightly if using different units (e.g., millimeters instead of centimeters), but this calculator uses the standard formula with radius in cm.
Q5: Can I use this calculator for any type of centrifuge?
A: Yes, this formula is universally applicable to all centrifuges, but always verify the specific requirements of your experimental protocol.